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How to Isolate High-Quality CD4+ T Cells for Reliable Immunology Research

2026.02.13

CD4+ T cells are central commanders of the adaptive immune system, critical for research in immunotherapy, autoimmune diseases, and cancer immunology. The success of these studies fundamentally depends on the initial quality of the isolated cells. Therefore, isolating CD4+ T cells that are not only pure but also highly viable and functionally intact is a crucial first step.

The common challenge lies in achieving a balance between three key parameters: purity, viability, and functional recovery. Many isolation methods can compromise one aspect for another. For example, aggressive techniques may yield high purity but damage cell health, leading to poor viability and an inability to respond properly in downstream functional assays like activation, proliferation, or cytokine secretion. This directly affects the reliability and reproducibility of experimental data.

So, what defines a high-quality isolation? The outcome should deliver cells that meet three concurrent standards:

l  High Purity (>95%): To ensure the population is specific and free from interfering cell types.

l  High Viability (>95%): To guarantee cells are healthy and capable of subsequent culture or stimulation.

l  Preserved Functionality: To confirm that isolated cells retain their native biological activity, evidenced by robust proliferation and cytokine production upon activation.

MileCell MagSep CD4 MicroBeads are designed to meet this triple benchmark. Utilizing gentle, nanoscale magnetic labeling, the system minimizes non-specific binding and cellular stress. Validation data demonstrate consistent batch-to-batch performance, delivering CD4+ T cells with superior purity and viability that function comparably to competitor products in key activation and proliferation assays. 

Ready to streamline the start of your immunology research?

Discover the detailed performance data and specifications for MileCell MagSep CD4 MicroBeads to ensure your next experiment is built on a solid foundation.

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Product Information:

 

Cat.No.

Product Description

Size

Capacity

AB0001

CD4 MicroBeads, Human

2mL

1×109  PBMCs