2025.12.28
CD8+ T cells play critical roles across a spectrum of medical and scientific fields, with major applications spanning cancer immunotherapy, infectious disease management, research and treatment of autoimmune diseases, transplant immunology monitoring and regulation, and fundamental immunology research
Milecell MagSep™ CD8 MicroBeads, Human are superparamagnetic nanoparticles conjugated to monoclonal anti-human CD8 antibodies for in vitro positive selection or depletion of CD8+ T cells from mononuclear cells drived from peripheral blood Leukopak, cord blood, bone marrow, and other single-cell suspensions.
Key Advantages:
l Preserves native cell viability and high purity
l Validated for T cell proliferation and cytokine release assay.
l User-friendly workflow, optimized columns enable rapid, efficient separation
Figure 1. Fresh human peripheral blood mononuclear cells (PBMCs) from different donors were used as the starting material to isolate CD8+ T cells under identical conditions with Milecell MagSep™ CD8 MicroBeads and competitor products, assessing post-separation viability, purity, and recovery.
(A) Viability of Target Cells Post-Separation
(B) Recovery of Target Cells
(C) Purity of Target Cell Pre- and Post-Separation
Figure 2. The isolated CD8+ T cells were stimulated for 20 hours with an activation cocktail (PMA + Ionomycin). Cell culture supernatants were analyzed for IFN-γ secretion by ELISA, while cells were assessed for surface marker expression (CD25/CD69) by flow cytometry. MileCell MagSep™ CD8 MicroBeads demonstrated comparable performance to competitor products in two critical functional parameters: CD25/CD69 expression levels, and IFN-γ secretion capacity.
(A) CD25 Expression in Activated and Non-activated Cells After Separation
(B) CD69 Expression in Activated and Non-activated Cells After Separation
(C) IFN-γ Secretion Analysis in Activated and Non-activated Cells After Separation
Cat.No. | Product Description | Size | Capacity |
AB0002 | CD8 MicroBeads, Human | 2mL | 1×109 PBMCs |
Contact:
For inquiries regarding our products, services, or technical assistance, please contact: Info@milecell-bio.com
