2025.12.21
CD4+ T cells play a central role in coordinating immune responses and are indispensable across multiple research fields, including immunotherapy, autoimmune disease research, and cancer immunology.
Milecell MagSep™ CD4 MicroBeads, Human are superparamagnetic nanoparticles conjugated to monoclonal anti-human CD4 antibodies for in vitro positive selection or depletion of CD4+ cells from PBMCs, Leukopak, cord blood, bone marrow, and other single-cell suspensions.
Key Advantages:
• High specificity with minimal non-specific binding
• Excellent batch-to-batch consistency
• Preserves native cell viability and functionality
Figure 1. Fresh human peripheral blood mononuclear cells (PBMCs) derived from 3 donors served as the starting material to compare CD4+ T cell isolation performance between Milecell MagSep™ CD4 MicroBeads and competitor products under identical conditions, assessing post-separation viability, purity, and recovery .
(A) Viability of Target Cells Post-Separation
(B) Recovery of Target Cells
(C) Purity of Target Cell Pre- and Post-Separation
Figure 2. The isolated CD4+ T cells were stimulated for 20 hours with an activation cocktail (PMA + Ionomycin). Cell culture supernatants were analyzed for IL-2 secretion by ELISA, while cells were assessed for surface marker expression (CD25/CD69) by flow cytometry. MileCell MagSep™ CD4 MicroBeads demonstrated comparable performance to competitor products in two critical functional parameters: CD25/CD69 expression levels, and IL-2 secretion capacity.
(A) CD25 Expression in Activated and Non-activated Cells After Separation
(B) CD69 Expression in Activated and Non-activated Cells After Separation
(C) IL-2 Secretion Analysis in Activated and Non-activated Cells After Separation
Cat.No. | Product Description | Size | Capacity |
AB0001 | CD4 MicroBeads, Human | 2mL | 1×109 PBMCs |
For inquiries regarding our products, services, or technical assistance, please contact: Info@milecell-bio.com
