2025.09.28
Still burning the midnight oil with PPTs and bubble tea at 2 AM? Beware! Your liver’s squad of "angry little guardians" is working overtime—they’re none other than the legendary Kupffer Cells (KCs)[1]!
Liver’s Immune Sentinels: Decoding Kupffer Cells
As resident liver macrophages, KCs:
l Constitute 2-5% of total hepatic cells & 15-20% of non-parenchymal cells
l Form the body’s largest fixed macrophage population along liver sinusoids
l Continuously phagocytize bacteria, dead cells, and toxins
l Serve as "immune sentries" regulating liver homeostasis[2]
In healthy livers, KCs:
√ Express low-level MHC II and costimulatory molecules
√ Suppress T-cell activation under normal conditions
KC Dysfunction: Gateway to Liver Disease
When injured hepatocytes release DAMPs (Damage-Associated Molecular Patterns):
→ KCs unleash pro-inflammatory cytokines/chemokines
→ Recruit monocyte-derived macrophages (MDMs)
→ Drive macrophage polarization linked to NAFLD/NASH progression[3]
√ Primary Isolation: Directly from animal livers
√ Purity Validation: Anti-CD68 immunofluorescence staining
√ Functional Highlights:
l Adherent growth with irregular stellate/polygonal morphology
l Visible pseudopods and phagocytic granules (e.g., ingested fluorescent microspheres)
l Limited proliferation in vitro, requiring M-CSF for sustained activity
√ Functional Verification:
LPS stimulation → ELISA-confirmed IL-6 secretion
Lot | Product | Specification |
CCD-KC | Cryopreserved Mouse KCs (Male, CD-1) | 0.5M |
CSD-KC | Cryopreserved Rat KCs (Male, SD) | 0.5M |
CBD-KC | Cryopreserved Canine KCs (Male, Beagle) | 0.5M |
CCY-KC | Cryopreserved Monkey KCs (Male, Cynomolgus) | 0.5M |
CBM-KC | Cryopreserved Minipig KCs (Male, Bama) | 0.5M |
* Custom species models available upon request
References
