2025.07.28
In the fast-paced world of drug discovery, reliable and physiologically relevant models are paramount for predicting human metabolic outcomes. Cryopreserved primary hepatocytes have emerged as a gold standard, offering unparalleled advantages for ADME/Tox studies. By optimizing cryopreservation techniques, these cells maintain ≥80% viability and retain >60% of the metabolic capacity of freshly isolated hepatocytes, making them ideal for short-term metabolism, toxicity, and mutagenicity testing.
Key Benefits:
l Species Versatility: Human, monkey, dog, rat, and mouse hepatocytes demonstrate consistent metabolic functions, including Phase I (e.g., CYP450 activities) and Phase II (e.g., UGT, GST) metabolism.
l Enhanced Reproducibility: Large pooled lots minimize donor variability, ensuring reliable inter-lot consistency for long-term studies.
l Critical Applications: Evaluate metabolic stability, enzyme induction/inhibition, transporter interactions, and hepatotoxicity in suspension or plated formats.
l Streamlined Workflows: Cryopreservation eliminates dependency on fresh tissue, enabling "on-demand" access and reducing experimental timelines.
Innovations Driving Success:
Advanced protocols, such as Percoll centrifugation for removing non-viable cells and computer-controlled freezing, preserve cofactors like NADPH and enhance functionality. For instance, Gibco™ and MileCell Bio offer validated hepatocytes with pre-qualified metabolic and transporter activities, supporting complex assays like benzo[a]pyrene metabolite profiling and induction studies.
Why Choose Cryopreserved Hepatocytes?
They bridge the gap between in vitro data and in vivo relevance, providing high predictability for clinical outcomes. As highlighted in recent studies, cryopreserved hepatocytes correlate strongly with fresh cells in metabolic clearance predictions, enabling confident decision-making in lead optimization.
Embrace the future of ADME/Tox testing—leveraging cryopreserved hepatocytes accelerates drug development while ensuring physiological accuracy.
Tags:
#DrugMetabolism #ADMETox #PharmaInnovation #Hepatocytes #DrugDiscovery #PreclinicalResearch #Biotech #LifeSciences #CRO #Pharmaceuticals
