2024.11.15
Key Applications of Liver Microsomes / S9 fractions in Drug Preclinical Studies.
Application | Description |
Metabolism Studies | Assess the metabolic stability of drugs and drug candidates to predict their metabolic fate in vivo. |
Metabolite Identification | Identify metabolites generated during drug metabolism to understand potential bioactivation or detoxification. |
Drug-Drug Interaction (DDI) Studies | Evaluate potential drug-drug interactions by assessing inhibition or induction of drug-metabolizing enzymes. |
Enzyme Kinetics Studies | Study enzyme-substrate binding, activity, and determine kinetic parameters such as Km and Vmax. |
Cytochrome P450 (CYP) Phenotyping | Determine activity and selectivity of specific CYP enzymes in drug metabolism, aiding in understanding variability. |
Drug Metabolism Prediction | Predict in vivo drug metabolism based on in vitro metabolic data to optimize drug candidates. |
Toxicity Screening | Assess potential for drug-induced toxicity, including hepatotoxicity, by studying formation of reactive metabolites. |
Bioavailability Prediction | Predict oral bioavailability of drugs by assessing metabolic stability and extent of first-pass metabolism. |
FDA / EMA Guidance Documents | |
Guidance for Industry: Drug Metabolism/Drug Interaction Studies in the Drug Development Process In Vitro Drug Interaction Studies —Cytochrome P450 Enzyme- and Transporter-Mediated Drug Interactions Safety Testing of Drug Metabolites Guidance for Industry Guideline on the investigation of drug interactions (European Medicines Agency) | |
Metabolic Stability in Animal Liver S9 Fractions
Milecell Bio’s animal liver S9 fractions are characterized by their enzymatic activity, protein content, and ability to metabolize various substrates. These fractions are obtained from different species, such as rats, mice and dogs, providing comparative insights into interspecies differences in drug metabolism. Our liver S9 fractions demonstrate excellent quality compared to well-known branded products, as evidenced by comparative metabolic stability
Drug Clearance Rate of Beagle Dog Liver S9 Batch V099 of MileCell Bio has either higher or comparableclearance rates for testosterone, verapamil, and raloxifenecompared to two well-known brands.
Drug Clearance Rate of Beagle Dog Liver S9 Batch V099 of MileCell Bio has either higher or comparableclearance rates for testosterone, verapamil, and raloxifenecompared to two well-known brands.
Drug Clearance Rate of CD-1 Mouse Liver S9 Fractions Batch CD-088 of MileCell Bio shows comparableclearance rates for testosterone, verapamil, and raloxifenecompared to three well-known brands.
